Human Renin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.08 pg/ml (50 μl)；0.25 pg/ml (10 μl)； |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0078 | 0.0077 | 0.0077 | |
| 2.74 | 0.0338 | 0.0321 | 0.0330 | 0.0253 |
| 8.23 | 0.0883 | 0.0978 | 0.0931 | 0.0854 |
| 24.69 | 0.2698 | 0.2577 | 0.2638 | 0.2561 |
| 74.07 | 0.7291 | 0.6849 | 0.7070 | 0.6993 |
| 222.22 | 1.7700 | 1.6890 | 1.7295 | 1.7218 |
| 666.67 | 3.1820 | 3.0870 | 3.1345 | 3.1268 |
| 2000.00 | 4.1599 | 4.1959 | 4.1779 | 4.1702 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 36.2 | 188.8 | 754.8 | 37.5 | 191.8 | 651.7 |
| Standard Deviation | 1.0 | 8.4 | 29.6 | 1.7 | 5.8 | 17.0 |
| Coefficient of Variation(%) | 2.8 | 4.4 | 3.9 | 4.6 | 3.0 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Renin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 106%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 415.85-1298.17 | 100 | 791.38 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Renin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Renin
Renin is a secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of Renin. The 406 aa precursor includes a signal peptide (aa 1 - 23), a propeptide (aa 24 - 66), and a mature chain (aa 67 - 406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.
Mouse Renin is a secreted, 42 - 47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin-1) is synthesized as a 381 aa proform (aa 22 - 402). In the kidney, prorenin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro-renin shares 70% and 85% aa sequence identity with human and rat pro-renin, respectively.
