Human CD273/B7-DA/PD-L2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 ng/ml (50 μl)；0.10 ng/ml (10 μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0077 | 0.0086 | 0.0082 | |
| 0.14 | 0.0225 | 0.0239 | 0.0232 | 0.0151 |
| 0.41 | 0.0562 | 0.0586 | 0.0574 | 0.0493 |
| 1.23 | 0.1686 | 0.1711 | 0.1699 | 0.1617 |
| 3.70 | 0.5579 | 0.5243 | 0.5411 | 0.5330 |
| 11.11 | 1.6230 | 1.5880 | 1.6055 | 1.5974 |
| 33.33 | 3.4830 | 3.5050 | 3.4940 | 3.4859 |
| 100.00 | 4.3062 | 4.3968 | 4.3515 | 4.3434 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 2.0 | 10.6 | 29.4 | 1.8 | 10.1 | 29.2 |
| Standard Deviation | 0.1 | 0.6 | 1.4 | 0.1 | 0.5 | 1.5 |
| Coefficient of Variation(%) | 2.7 | 5.3 | 4.9 | 5.2 | 5.4 | 5.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD273/B7-DC/PD-L2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 120% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.48-15.01 | 100 | 9.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD273/B7-DC/PD-L2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD273/B7-DA/PD-L2
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.
