Human SNAP25 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.18 pg/ml (50 μl)；2.61 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0086 | 0.0097 | 0.0092 | |
| 6.86 | 0.0264 | 0.0274 | 0.0269 | 0.0178 |
| 20.58 | 0.0653 | 0.0679 | 0.0666 | 0.0575 |
| 61.73 | 0.1779 | 0.1843 | 0.1811 | 0.1720 |
| 185.19 | 0.5479 | 0.5375 | 0.5427 | 0.5336 |
| 555.56 | 1.4790 | 1.4530 | 1.4660 | 1.4569 |
| 1666.67 | 3.2017 | 3.1982 | 3.2000 | 3.1908 |
| 5000.00 | 4.3728 | 4.4603 | 4.4166 | 4.4074 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 97.4 | 500.6 | 1577.5 | 98.0 | 532.5 | 1626.4 |
| Standard Deviation | 5.8 | 31.3 | 86.8 | 3.8 | 34.7 | 93.8 |
| Coefficient of Variation(%) | 6.0 | 6.2 | 5.5 | 3.9 | 6.5 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human SNAP25 into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 97% to 106% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.30 | 65 | 1.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SNAP25 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: SNAP25
SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. SNAP25 is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems and it associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein. This antibody is particularly valuable for immunocytochemical studies of the human brain due to its stability in formalin sections.
