Human ICAM-1/CD54 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.22 pg/ml (50 μl)；0.05 ng/ml (10 μl) |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 75min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0050 | 0.0046 | 0.0048 | |
| 0.14 | 0.0210 | 0.0195 | 0.0203 | 0.0155 |
| 0.41 | 0.0519 | 0.0540 | 0.0530 | 0.0482 |
| 1.23 | 0.1489 | 0.1465 | 0.1477 | 0.1429 |
| 3.70 | 0.4238 | 0.4106 | 0.4172 | 0.4124 |
| 11.11 | 1.0740 | 1.0970 | 1.0855 | 1.0807 |
| 33.33 | 2.3350 | 2.3240 | 2.3295 | 2.3247 |
| 100.00 | 3.1570 | 3.3020 | 3.2295 | 3.2247 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.8 | 9.0 | 32.7 | 1.7 | 8.7 | 31.0 |
| Standard Deviation | 0.1 | 0.4 | 2.2 | 0.1 | 0.4 | 1.0 |
| Coefficient of Variation(%) | 3.3 | 4.8 | 6.8 | 5.8 | 4.3 | 3.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human ICAM-1/CD54 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 120% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 55.22-176.34 | 100.0 | 103.75 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of ICAM-1/CD54 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.
