Human IL-18BP enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.67 pg/ml (50 μl)；1.70 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0229 | 0.0213 | 0.0221 | |
| 13.72 | 0.0390 | 0.0375 | 0.0383 | 0.0162 |
| 41.15 | 0.0764 | 0.0680 | 0.0722 | 0.0501 |
| 123.46 | 0.1688 | 0.1584 | 0.1636 | 0.1415 |
| 370.37 | 0.4373 | 0.4087 | 0.4230 | 0.4009 |
| 1111.11 | 1.1060 | 1.0640 | 1.0850 | 1.0629 |
| 3333.33 | 2.4970 | 2.4830 | 2.4900 | 2.4679 |
| 10000.00 | 3.7010 | 3.7690 | 3.7350 | 3.7129 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 230.8 | 939.3 | 3046.5 | 213.2 | 861.2 | 2715.9 |
| Standard Deviation | 8.9 | 64.3 | 188.3 | 14.1 | 48.6 | 71.8 |
| Coefficient of Variation(%) | 3.8 | 6.8 | 6.2 | 6.6 | 5.6 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-18BP into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 106% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 22.78-56.27 | 100 | 32.77 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-18BP in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: IL-18BP
Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.
