Human IL-1β enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.77 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0243 | 0.0232 | 0.0238 | |
| 1.37 | 0.0294 | 0.0277 | 0.0286 | 0.0048 |
| 4.12 | 0.0396 | 0.0382 | 0.0389 | 0.0152 |
| 12.35 | 0.078 | 0.0693 | 0.0737 | 0.0499 |
| 37.04 | 0.171 | 0.1696 | 0.1703 | 0.1466 |
| 111.11 | 0.4662 | 0.4216 | 0.4439 | 0.4202 |
| 333.33 | 1.1176 | 1.0011 | 1.0594 | 1.0356 |
| 1000 | 2.4482 | 2.2666 | 2.3574 | 2.3337 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 19.2 | 97.8 | 341.4 | 20.3 | 107.9 | 346.9 |
| Standard Deviation | 0.5 | 6.1 | 23.7 | 0.7 | 3.6 | 13.8 |
| Coefficient of Variation(%) | 2.8 | 6.2 | 6.9 | 3.5 | 3.3 | 4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-1 beta into health human serum sample. The un-spiked serum was used as blank in these experiments.
The recovery ranged from 113% to 137% with an overall mean recovery of 125%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-104.5 | 78 | 12.27 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-10 in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-1β
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
