Mouse Leptin DTSet enzyme-linked immunoassay kit
| Specification | 96*5 Test；96T*15 Test |
|---|---|
| Standard Curve Range | 125 pg/ml-8000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Not Ready-to-Use |
| Test Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0333 | 0.0260 | 0.0297 | |
| 125.00 | 0.1279 | 0.1226 | 0.1253 | 0.0956 |
| 250.00 | 0.2287 | 0.2357 | 0.2322 | 0.2026 |
| 500.00 | 0.4336 | 0.4045 | 0.4191 | 0.3894 |
| 1000.00 | 0.7352 | 0.7579 | 0.7466 | 0.7169 |
| 2000.00 | 1.2960 | 1.4160 | 1.3560 | 1.3264 |
| 4000.00 | 2.4040 | 2.4790 | 2.4415 | 2.4119 |
| 8000.00 | 4.0328 | 3.8810 | 3.9569 | 3.9273 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DTSet Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: YOUKE Life, Catalog # DSEP01. Plate Sealers: YOUKE Life, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . YOUKE Life, Catalog # DSCB01.
- Blocking Buffer: YOUKE Life, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. YOUKE Life, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.YOUKE Life, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. YOUKE Life, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. YOUKE Life, Catalog # DSSS01.
Product Data Sheet
Background: Leptin
Leptin (anti-obesity protein) is a protein product of the mouse ob (obesity) gene and appears to be involved in appetite control. The ob gene is expressed in adipose tissue and is believed to regulate fat storage in the human body. Mice with ob/ob genotype also develop a form of diabetes similar to type II (non-insulin-dependent) diabetes. Mouse leptin is a 16 kDa protein containing 147 amino acid residues.
