Human VCAM-1/CD106 DTSet enzyme-linked immunoassay kit
| Specification | 96*5 Test；96T*15 Test |
|---|---|
| Standard Curve Range | 156.25pg/ml-10000pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Not Ready-to-Use |
| Test Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0262 | 0.0284 | 0.0273 | |
| 156.25 | 0.1685 | 0.1729 | 0.1707 | 0.1434 |
| 312.50 | 0.3000 | 0.2813 | 0.2907 | 0.2634 |
| 625.00 | 0.5450 | 0.4874 | 0.5162 | 0.4889 |
| 1250.00 | 0.8849 | 0.9916 | 0.9383 | 0.9110 |
| 2500.00 | 1.6470 | 1.6690 | 1.6580 | 1.6307 |
| 5000.00 | 2.6060 | 2.5700 | 2.5880 | 2.5607 |
| 10000.00 | 4.0800 | 3.7370 | 3.9085 | 3.8812 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DTSet Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: YOUKE Life, Catalog # DSEP01. Plate Sealers: YOUKE Life, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2μm filtered . YOUKE Life, Catalog # DSCB01.
- Blocking Buffer: YOUKE Life, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween® 20 in PBS, pH 7.2-7.4. YOUKE Life, Catalog # DSWB01.
- Assay Buffer: 0.5% BSA，0.05% Tween® 20，PBS Solution.YOUKE Life, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. YOUKE Life, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. YOUKE Life, Catalog # DSSS01.
Product Data Sheet
Background: VCAM-1/CD106
VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.
