Human B7-H2/CD275 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.01 pg/ml (50 μl)；4.95 pg/ml (10 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0144 | 0.0126 | 0.0135 | |
| 13.72 | 0.0336 | 0.0343 | 0.0340 | 0.0205 |
| 41.15 | 0.0807 | 0.0725 | 0.0766 | 0.0631 |
| 123.46 | 0.1894 | 0.2274 | 0.2084 | 0.1949 |
| 370.37 | 0.4951 | 0.5551 | 0.5251 | 0.5116 |
| 1111.11 | 1.2880 | 1.3600 | 1.3240 | 1.3105 |
| 3333.33 | 2.7260 | 2.8360 | 2.7810 | 2.7675 |
| 10000.00 | 4.1722 | 4.2258 | 4.1990 | 4.1855 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 246.6 | 1218.8 | 4221.3 | 132.7 | 829.4 | 3607.5 |
| Standard Deviation | 19.3 | 97.6 | 239.4 | 5.4 | 63.9 | 268.3 |
| Coefficient of Variation(%) | 7.8 | 8.0 | 5.7 | 4.0 | 7.7 | 7.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human B7-H2/CD275 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 72% to 107% with an overall mean recovery of 87%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.07-193.97 | 100 | 106.56 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of B7-H2/CD275 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: B7-H2/CD275
ICOSL protein (Inducible T-cell COStimulator Ligand), also known as B7h or CD275 protein, is a ligand of ICOS and belongs to one of the members of the B7 family. ICOSL is mainly expressed on the surface of antigen-presenting cells such as dendritic cells and B cells. The binding of ICOSL to ICOS can provide costimulatory signals that promote activation, proliferation and differentiation of T cells.
