Human cIAP2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.81 pg/ml (50 μl)；1.59 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0145 | 0.0142 | |
| 2.74 | 0.0221 | 0.0234 | 0.0228 | 0.0086 |
| 8.23 | 0.0328 | 0.0302 | 0.0315 | 0.0173 |
| 24.69 | 0.0653 | 0.0689 | 0.0671 | 0.0529 |
| 74.07 | 0.1791 | 0.1718 | 0.1755 | 0.1613 |
| 222.22 | 0.5172 | 0.5250 | 0.5211 | 0.5069 |
| 666.67 | 1.4860 | 1.4850 | 1.4855 | 1.4713 |
| 2000.00 | 3.3930 | 3.4200 | 3.4065 | 3.3923 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 27.3 | 173.6 | 525.6 | 39.3 | 185.7 | 604.2 |
| Standard Deviation | 2.1 | 11.6 | 25.5 | 3.1 | 13.1 | 37.2 |
| Coefficient of Variation(%) | 7.9 | 6.7 | 4.9 | 8.0 | 7.1 | 6.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human cIAP2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 119% with an overall mean recovery of 110%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-4.39 | 33.3 | 1.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of cIAP2 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: cIAP2
Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.
