Human Granzyme A/GZMA enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.46 pg/ml (50 μl)；1.46 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0136 | 0.0141 | 0.0139 | |
| 2.74 | 0.0255 | 0.0239 | 0.0247 | 0.0109 |
| 8.23 | 0.0392 | 0.0410 | 0.0401 | 0.0263 |
| 24.69 | 0.0845 | 0.0824 | 0.0835 | 0.0696 |
| 74.07 | 0.2141 | 0.2112 | 0.2127 | 0.1988 |
| 222.22 | 0.6253 | 0.5911 | 0.6082 | 0.5944 |
| 666.67 | 1.7910 | 1.7440 | 1.7675 | 1.7537 |
| 2000.00 | 3.7830 | 3.7900 | 3.7865 | 3.7727 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 49.6 | 198.7 | 609.8 | 47.5 | 189.0 | 608.9 |
| Standard Deviation | 2.3 | 8.7 | 26.3 | 1.5 | 7.3 | 20.0 |
| Coefficient of Variation(%) | 4.6 | 4.4 | 4.3 | 3.2 | 3.9 | 3.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Granzyme A into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 97% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 62.47-179.55 | 100.0 | 113.58 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Granzyme A in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Granzyme A/GZMA
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
