Human B7-H2/CD275 DTSet enzyme-linked immunoassay kit
| Specification | 96*5 Test；96T*15 Test |
|---|---|
| Standard Curve Range | 31.25 pg/ml -2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Not Ready-to-Use |
| Test Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0444 | 0.0391 | 0.0418 | |
| 31.25 | 0.1922 | 0.1449 | 0.1686 | 0.1268 |
| 62.50 | 0.3485 | 0.2536 | 0.3011 | 0.2593 |
| 125.00 | 0.5845 | 0.4568 | 0.5207 | 0.4789 |
| 250.00 | 0.9498 | 0.7517 | 0.8508 | 0.8090 |
| 500.00 | 1.5070 | 1.1090 | 1.3080 | 1.2663 |
| 1000.00 | 1.9830 | 1.7240 | 1.8535 | 1.8118 |
| 2000.00 | 2.4640 | 2.1900 | 2.3270 | 2.2853 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DTSet Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: YOUKE Life, Catalog # DSEP01. Plate Sealers: YOUKE Life, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . YOUKE Life, Catalog # DSCB01.
- Blocking Buffer: YOUKE Life, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. YOUKE Life, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.YOUKE Life, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. YOUKE Life, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. YOUKE Life, Catalog # DSSS01.
Product Data Sheet
Background: B7-H2/CD275
ICOSL protein (Inducible T-cell COStimulator Ligand), also known as B7h or CD275 protein, is a ligand of ICOS and belongs to one of the members of the B7 family. ICOSL is mainly expressed on the surface of antigen-presenting cells such as dendritic cells and B cells. The binding of ICOSL to ICOS can provide costimulatory signals that promote activation, proliferation and differentiation of T cells.
