Mouse/Rat GDF-15 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.20 pg/ml (10 μl) |
| Standard Curve Range | 4.10~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0175 | 0.0162 | 0.0169 | |
| 4.10 | 0.0347 | 0.0336 | 0.0342 | 0.0173 |
| 10.24 | 0.0514 | 0.0499 | 0.0507 | 0.0338 |
| 25.60 | 0.1131 | 0.1059 | 0.1095 | 0.0927 |
| 64.00 | 0.2521 | 0.2470 | 0.2496 | 0.2327 |
| 160.00 | 0.6446 | 0.5856 | 0.6151 | 0.5983 |
| 400.00 | 1.4250 | 1.4270 | 1.4260 | 1.4092 |
| 1000.00 | 2.9220 | 2.9260 | 2.9240 | 2.9072 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 38.8 | 143.9 | 357.6 | 39.0 | 143.8 | 359.6 |
| Standard Deviation | 1.0 | 3.0 | 10.8 | 1.0 | 2.5 | 7.7 |
| Coefficient of Variation(%) | 2.4 | 2.1 | 3.0 | 2.5 | 1.7 | 2.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse/Rat GDF-15 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 125% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 12.57-33.73 | 100 | 20.84 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of GDF-15 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: GDF-15
GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.
