Human CD276/B7-H3 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.34 pg/ml (50 μl)；1.64 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0245 | 0.0188 | 0.0217 | |
| 6.86 | 0.0357 | 0.0299 | 0.0328 | 0.0112 |
| 20.58 | 0.0549 | 0.0489 | 0.0519 | 0.0303 |
| 61.73 | 0.1073 | 0.1138 | 0.1106 | 0.0889 |
| 185.19 | 0.2891 | 0.2753 | 0.2822 | 0.2606 |
| 555.56 | 0.7364 | 0.7668 | 0.7516 | 0.7300 |
| 1666.67 | 1.7640 | 1.6930 | 1.7285 | 1.7069 |
| 5000.00 | 3.2980 | 3.2950 | 3.2965 | 3.2749 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 100.5 | 499.3 | 1636.1 | 93.4 | 457.0 | 1443.3 |
| Standard Deviation | 5.1 | 22.3 | 81.7 | 4.2 | 18.2 | 4.0 |
| Coefficient of Variation(%) | 5.1 | 4.5 | 5.0 | 4.5 | 4.0 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD276/B7-H3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.04-9.65 | 100.0 | 6.68 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD276/B7-H3 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD276/B7-H3
B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.
