Human Adiponectinenzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.14 ng/ml (10 μl) |
| Standard Curve Range | 15.63~1000 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0079 | 0.0072 | 0.0076 | |
| 15.63 | 0.0964 | 0.0988 | 0.0976 | 0.0901 |
| 31.25 | 0.1636 | 0.1991 | 0.1814 | 0.1738 |
| 62.50 | 0.3482 | 0.3617 | 0.3550 | 0.3474 |
| 125.00 | 0.7514 | 0.7524 | 0.7519 | 0.7444 |
| 250.00 | 1.5100 | 1.5200 | 1.5150 | 1.5075 |
| 500.00 | 2.0590 | 2.4110 | 2.2350 | 2.2275 |
| 1000.00 | 3.6840 | 3.3470 | 3.5155 | 3.5080 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 13.5 | 75.9 | 218.3 | 16.5 | 79.5 | 230.5 |
| Standard Deviation | 0.9 | 5.8 | 13.3 | 1.2 | 5.7 | 9.6 |
| Coefficient of Variation(%) | 6.7 | 7.6 | 6.1 | 7.1 | 7.1 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Adiponectin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 119% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.63-6.61 | 100 | 3.93 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Adiponectin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Adiponectin
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
