Mammal VEGF-C enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.82 pg/ml (50 μl) |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0193 | 0.0193 | 0.0193 | |
| 13.72 | 0.0278 | 0.0271 | 0.0275 | 0.0082 |
| 41.15 | 0.0444 | 0.0434 | 0.0439 | 0.0246 |
| 123.46 | 0.0984 | 0.0931 | 0.0958 | 0.0765 |
| 370.37 | 0.2400 | 0.2467 | 0.2434 | 0.2241 |
| 1111.11 | 0.6659 | 0.6791 | 0.6725 | 0.6532 |
| 3333.33 | 1.7940 | 1.8210 | 1.8075 | 1.7882 |
| 10000.00 | 3.8780 | 3.7850 | 3.8315 | 3.8122 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 240.1 | 1112.3 | 3324.8 | 233.3 | 1079.7 | 3258.5 |
| Standard Deviation | 12.3 | 50.9 | 159.1 | 7.2 | 37.5 | 71.2 |
| Coefficient of Variation(%) | 5.1 | 4.6 | 4.8 | 3.1 | 3.5 | 2.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mammal VEGF-C into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 110% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 474.36-2997.14 | 100 | 1487.71 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VEGF-C in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: VEGF-C
Vascular endothelial growth factor C (VEGF-C) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-C containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. Both the ligand and receptor are usually co-expressed at sites of lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, neoangiogenesis, and vessel sprouting, resulting in enhanced lymph flow and metastasis to regional lymph nodes.
