Human CCL23/MPIF-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.10 pg/ml (50 μl)；1.66 pg/ml (10 μl) |
| Standard Curve Range | 4.1~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0095 | 0.0087 | 0.0091 | |
| 4.10 | 0.0230 | 0.0207 | 0.0219 | 0.0128 |
| 10.24 | 0.0500 | 0.0457 | 0.0479 | 0.0388 |
| 25.60 | 0.1250 | 0.1321 | 0.1286 | 0.1195 |
| 64.00 | 0.3903 | 0.3985 | 0.3944 | 0.3853 |
| 160.00 | 1.3330 | 1.2530 | 1.2930 | 1.2839 |
| 400.00 | 3.3930 | 3.7000 | 3.5465 | 3.5374 |
| 1000.00 | 4.3920 | 4.4231 | 4.4076 | 4.3985 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 19.9 | 99.0 | 302.5 | 21.6 | 107.5 | 319.6 |
| Standard Deviation | 1.2 | 3.9 | 9.7 | 1.2 | 4.1 | 13.2 |
| Coefficient of Variation(%) | 5.8 | 3.9 | 3.2 | 5.6 | 3.8 | 4.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL23/MPIF-1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 120% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 131.30-479.56 | 100.0 | 371.04 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CCL23/MPIF-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CCL23/MPIF-1
Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.
