Human TREM-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.00 pg/ml (50 μl)；2.61 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0065 | 0.0073 | |
| 13.72 | 0.0150 | 0.0136 | 0.0143 | 0.0070 |
| 41.15 | 0.0331 | 0.0318 | 0.0325 | 0.0252 |
| 123.46 | 0.0905 | 0.0925 | 0.0915 | 0.0842 |
| 370.37 | 0.2612 | 0.2627 | 0.2620 | 0.2547 |
| 1111.11 | 0.8480 | 0.8700 | 0.8590 | 0.8517 |
| 3333.33 | 2.7722 | 2.7375 | 2.7549 | 2.7476 |
| 10000.00 | 4.5294 | 4.6098 | 4.5696 | 4.5623 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 191.5 | 997.5 | 2959.8 | 190.9 | 991.8 | 2942.6 |
| Standard Deviation | 6.9 | 41.5 | 167.5 | 7.5 | 23.5 | 150.3 |
| Coefficient of Variation(%) | 3.6 | 4.2 | 5.7 | 3.9 | 2.4 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human TREM-1 into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 113% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 92.32-631.59 | 100 | 249.37 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TREM-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TREM-1
TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with
LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology.
