Human CD254/RANKL/TNFSF11 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.20 pg/ml (50 μl)；0.76 pg/ml (10 μl)； |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0117 | 0.0115 | |
| 6.86 | 0.0282 | 0.0296 | 0.0289 | 0.0174 |
| 20.58 | 0.0583 | 0.0597 | 0.0590 | 0.0475 |
| 61.73 | 0.1615 | 0.1627 | 0.1621 | 0.1506 |
| 185.19 | 0.4068 | 0.4528 | 0.4298 | 0.4183 |
| 555.56 | 1.0560 | 1.1330 | 1.0945 | 1.0830 |
| 1666.67 | 2.2150 | 2.1870 | 2.2010 | 2.1895 |
| 5000.00 | 3.2400 | 3.2890 | 3.2645 | 3.2530 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 98.6 | 567.8 | 1800.9 | 81.2 | 446.1 | 1484.8 |
| Standard Deviation | 5.2 | 41.2 | 140.4 | 3.2 | 13.9 | 70.5 |
| Coefficient of Variation(%) | 5.3 | 7.3 | 7.8 | 3.9 | 3.1 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Human CD254/RANKL/TNFSF11 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 102% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 0.63-9.14 | 100 | 4.14 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD254/RANKL/TNFSF11 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CD254/RANKL/TNFSF11
RANKL (TNFSF11) 是一种 II 型跨膜蛋白，是 NF-κB (RANK) 受体的配体。RANKL 是 NF-κB 的激活剂。RANKL 可与 NF-κB 结合并诱导单核细胞/巨噬细胞谱系细胞分化为破骨细胞，进一步导致破骨细胞前体成熟。因此，RANKL 对于破骨细胞成熟、骨骼建模和骨骼重塑，以及淋巴结 (LN) 的发育至关重要。在骨组织中，RANKL 由成骨细胞、骨细胞和免疫细胞表达，尤其是成骨细胞和骨细胞中[1]。人 RANKL 与小鼠和大鼠的氨基酸同源性分别为 82.02% 和 84.44%。小鼠 RANKL 与大鼠的氨基酸同源性为 94.94%。人 RANKL 由细胞质结构域 (M1-S47)、螺旋结构域 (M48-F68) 和细胞外结构域 (Y69-D317) 组成。可溶性片段 (140-317) 在被基质金属蛋白酶 (MMP3 或 7) 和 ADAM 等酶切割后可被释放。
