Human CD254/RANKL/TNFSF11 ELISA Kit检测试剂盒
| 规格 | 96 Test |
|---|---|
| 灵敏度 | 0.20 pg/ml (50 μl)；0.76 pg/ml (10 μl)； |
| 标准曲线范围 | 6.86~5000 pg/ml |
| 标准曲线梯度 | 7点/3倍 |
| 孵育次数 | 2 |
| 可检测样本 | 可溶性物质的液相样品。例如：血清，血浆，细胞培养上清，组织研磨液等。 |
| 加样体积 | 50 μl/10 μl |
| 类型 | 完全即用型 |
| 操作时长 | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0113 | 0.0117 | 0.0115 | |
| 6.86 | 0.0282 | 0.0296 | 0.0289 | 0.0174 |
| 20.58 | 0.0583 | 0.0597 | 0.0590 | 0.0475 |
| 61.73 | 0.1615 | 0.1627 | 0.1621 | 0.1506 |
| 185.19 | 0.4068 | 0.4528 | 0.4298 | 0.4183 |
| 555.56 | 1.0560 | 1.1330 | 1.0945 | 1.0830 |
| 1666.67 | 2.2150 | 2.1870 | 2.2010 | 2.1895 |
| 5000.00 | 3.2400 | 3.2890 | 3.2645 | 3.2530 |
精度
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 98.6 | 567.8 | 1800.9 | 81.2 | 446.1 | 1484.8 |
| Standard Deviation | 5.2 | 41.2 | 140.4 | 3.2 | 13.9 | 70.5 |
| Coefficient of Variation(%) | 5.3 | 7.3 | 7.8 | 3.9 | 3.1 | 4.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
加标回收
健康人血清加入 3 个不同浓度水平的人 CD254/RANKL/TNFSF11 ，未加人 CD254/RANKL/TNFSF11 的血清作为本底，计算回收率。回收率的范围从 80 % 至 102 %，平均回收率为 91%。
样本值
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 0.63-9.14 | 100 | 4.14 |
应用本试剂盒，检测 30 个健康志愿者的血清样本，志愿者的用药史不详。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
产品数据表
背景: CD254/RANKL/TNFSF11
RANKL (TNFSF11) is a type II transmembrane protein that is a ligand for the NF-κB (RANK) receptor. RANKL is an activator of NF-κB. RANKL binds to NF-κB and induces differentiation into osteoclasts in monocyte/macrophage lineage cells, further leading to osteoclast precursor maturation. Therefore, RANKL is essential for osteoclast maturation, bone modeling and bone remodeling, and lymph node (LN) development. In bone tissue, RANKL is expressed by osteoblasts, osteocytes, and immune cells, especially in osteoblasts and osteocytes [1]. Human RANKL had amino acid homology of 82.02% and 84.44% in mice and rats, respectively. The amino acid homology of mouse RANKL and rat was 94.94%. Human RANKL consists of a cytoplasmic domain (M1-S47), a helical domain (M48-F68), and an extracellular domain (Y69-D317). Soluble fragments (140-317) can be released after being cleaved by enzymes such as matrix metalloproteinases (MMP3 or 7) and ADAM.
