Human CCL18/PARC/MIP-4 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.13 pg/ml (50 μl)；7.26 pg/ml (10 μl)； |
| Standard Curve Range | 15.63 ~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0386 | 0.0375 | 0.0381 | |
| 15.63 | 0.1236 | 0.1275 | 0.1256 | 0.0875 |
| 31.25 | 0.2108 | 0.2100 | 0.2104 | 0.1724 |
| 62.50 | 0.3804 | 0.3785 | 0.3795 | 0.3414 |
| 125.00 | 0.6842 | 0.6825 | 0.6834 | 0.6453 |
| 250.00 | 1.2560 | 1.3060 | 1.2810 | 1.2430 |
| 500.00 | 2.1770 | 2.2180 | 2.1975 | 2.1595 |
| 1000.00 | 3.1250 | 3.1780 | 3.1515 | 3.1135 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 42.2 | 224.5 | 753.7 | 43.6 | 199.5 | 614.8 |
| Standard Deviation | 1.5 | 7.7 | 34.5 | 1.8 | 6.2 | 29.3 |
| Coefficient of Variation(%) | 3.5 | 3.4 | 4.6 | 4.1 | 3.1 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CCL18/PARC/MIP-4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 109% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 7.85-29.14 | 100 | 18.17 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human CCL18/PARC/MIP-4 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CCL18/PARC/MIP-4
Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.
