Serotonin/5-HT ELISA enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.60 ng/ml (100 μl)； |
| Standard Curve Range | 2.06~500 ng/ml |
| Standard Curve Gradient | 6 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 90min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 3.0250 | 3.0250 | 3.0800 | |
| 2.06 | 2.8490 | 2.9960 | 2.9225 | 2.9225 |
| 6.17 | 2.4040 | 2.5670 | 2.4855 | 2.4855 |
| 18.52 | 1.5540 | 1.7180 | 1.6360 | 1.6360 |
| 55.56 | 0.6366 | 0.7165 | 0.6766 | 0.6766 |
| 166.67 | 0.2532 | 0.2435 | 0.2484 | 0.2484 |
| 500.00 | 0.0608 | 0.0576 | 0.0592 | 0.0592 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 17.8 | 57.7 | 149.5 | 11.1 | 58.5 | 147.8 |
| Standard Deviation | 1.5 | 3.8 | 10.8 | 1.3 | 4.7 | 12.8 |
| Coefficient of Variation(%) | 8.2 | 6.6 | 7.2 | 12.1 | 8.0 | 8.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Serotonin/5-HT into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 121% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.13-67.10 | 100 | 31.68 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Serotonin/5-HT in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Serotonin/5-HT
5-hydroxytryptamine (5-HT) is an indole derivative, abbreviated as 5-HT, with the chemical formula C10H12N2O and a molecular weight of 176.22. It is an important neurotransmitter with a variety of physiological regulatory effects, belongs to the biogenic amine class, and is widely found in mammalian tissues. It plays a key regulatory role in both humans and animals, [1] particularly in the central nervous system. Serotonin is mainly found in platelets and neurons of the central nervous system, and is synthesized from tryptophan through multiple biosynthetic steps.
