Rat/Mouse IL-18 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 1.33 pg/ml (50 μl)；6.54 pg/ml (10 μl) |
| Standard Curve Range | 15.63 ~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0486 | 0.0516 | 0.0516 | |
| 15.63 | 0.1515 | 0.1558 | 0.1537 | 0.1021 |
| 31.25 | 0.2621 | 0.2558 | 0.2590 | 0.2074 |
| 62.50 | 0.4385 | 0.4635 | 0.4510 | 0.3994 |
| 125.00 | 0.8578 | 0.8121 | 0.8350 | 0.7834 |
| 250.00 | 1.4910 | 1.5100 | 1.5005 | 1.4489 |
| 500.00 | 2.6860 | 2.6880 | 2.6870 | 2.6354 |
| 1000.00 | 3.9985 | 4.1109 | 4.0547 | 4.0031 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 22.8 | 109.2 | 320.5 | 18.4 | 106.0 | 325.4 |
| Standard Deviation | 1.4 | 3.4 | 8.7 | 1.0 | 3.4 | 8.3 |
| Coefficient of Variation(%) | 5.9 | 3.1 | 2.7 | 5.5 | 3.2 | 2.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Rat/Mouse IL-18 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 77% to 119% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Rat Serum | 30 | 13.66-60.69 | 100 | 34.07 |
| Mouse Serum | 30 | n.d.-393.45 | 80 | 60.12 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Rat/Mouse IL-18 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-18
Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.
