Rat CXCL2/MIP-2 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.38 pg/ml (50 μl)；7.89 pg/ml (10 μl) |
| Standard Curve Range | 15.63 ~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0103 | 0.0099 | 0.0101 | |
| 15.63 | 0.0137 | 0.0143 | 0.0140 | 0.0039 |
| 31.25 | 0.0265 | 0.0243 | 0.0254 | 0.0153 |
| 62.50 | 0.0712 | 0.0657 | 0.0685 | 0.0584 |
| 125.00 | 0.2586 | 0.2330 | 0.2458 | 0.2357 |
| 250.00 | 1.0010 | 0.9430 | 0.9720 | 0.9619 |
| 500.00 | 2.5543 | 2.5634 | 2.5589 | 2.5488 |
| 1000.00 | 4.3521 | 4.4022 | 4.3772 | 4.3671 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 18.4 | 97.8 | 293.7 | 1.6 | 25.1 | 137.2 |
| Standard Deviation | 1.4 | 5.1 | 14.4 | 0.1 | 1.0 | 6.4 |
| Coefficient of Variation(%) | 7.8 | 5.1 | 4.9 | 6.9 | 4.0 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Rat CXCL2/MIP-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 75% to 125% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-22.05 | 75 | 11.87 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Rat CXCL2/MIP-2 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CXCL2/MIP-2
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
