Rat CXCL1/GRO alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.26 pg/ml (50 μl)；0.46 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0111 | 0.0132 | 0.0122 | |
| 1.37 | 0.0273 | 0.0238 | 0.0256 | 0.0134 |
| 4.12 | 0.0553 | 0.0516 | 0.0535 | 0.0413 |
| 12.35 | 0.1428 | 0.1471 | 0.1450 | 0.1328 |
| 37.04 | 0.4454 | 0.4437 | 0.4446 | 0.4324 |
| 111.11 | 1.1680 | 1.1480 | 1.1580 | 1.1459 |
| 333.33 | 2.3760 | 2.4200 | 2.3980 | 2.3859 |
| 1000.00 | 3.5740 | 3.6860 | 3.6300 | 3.6179 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 24.1 | 100.7 | 312.1 | 23.5 | 97.1 | 300.4 |
| Standard Deviation | 1.3 | 4.2 | 12.5 | 1.9 | 3.1 | 7.1 |
| Coefficient of Variation(%) | 4.5 | 3.3 | 4.6 | 3.4 | 4.1 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of rat CXCL1/GRO alpha into health rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 19.72-91.66 | 100 | 44.46 |
Serum/Plasma – Thirty samples from apparently healthy rats were evaluated for the presence of CXCL1/GRO in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: CXCL1/GRO
CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.
