Mouse Resistin enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.001 ng/ml (10 μl) |
| Standard Curve Range | 1.56~100 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0286 | 0.0296 | 0.0291 | |
| 1.56 | 0.0844 | 0.0949 | 0.0897 | 0.0606 |
| 3.13 | 0.1841 | 0.1639 | 0.1740 | 0.1449 |
| 6.25 | 0.3404 | 0.3503 | 0.3454 | 0.3163 |
| 12.50 | 0.6524 | 0.5893 | 0.6209 | 0.5918 |
| 25.00 | 1.1861 | 1.1035 | 1.1448 | 1.1157 |
| 50.00 | 2.3761 | 2.4024 | 2.3893 | 2.3602 |
| 100.00 | 3.9695 | 4.1788 | 4.0742 | 4.0451 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.9 | 10.5 | 28.4 | 1.9 | 9.3 | 28.6 |
| Standard Deviation | 0.1 | 0.7 | 1.6 | 0.1 | 0.6 | 1.0 |
| Coefficient of Variation(%) | 6.1 | 6.4 | 5.8 | 5.5 | 6.0 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Resistin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 105% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.23-18.70 | 100 | 11.5 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Resistin in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Resistin
Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.
