Mouse/Rat VEGFR3/FLT4 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.18 ng/ml (10 μl) |
| Standard Curve Range | 7.81~500 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0064 | 0.0066 | 0.0066 | |
| 7.81 | 0.0530 | 0.0511 | 0.0521 | 0.0455 |
| 15.63 | 0.1109 | 0.1045 | 0.1077 | 0.1011 |
| 31.25 | 0.2141 | 0.2223 | 0.2182 | 0.2116 |
| 62.50 | 0.4542 | 0.4671 | 0.4607 | 0.4541 |
| 125.00 | 0.9015 | 0.9226 | 0.9121 | 0.9055 |
| 250.00 | 1.8140 | 1.8360 | 1.8250 | 1.8184 |
| 500.00 | 3.1490 | 3.1270 | 3.1380 | 3.1314 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 10.0 | 47.4 | 152.3 | 10.4 | 48.9 | 160.6 |
| Standard Deviation | 0.5 | 1.7 | 5.2 | 0.5 | 1.6 | 7.3 |
| Coefficient of Variation(%) | 4.8 | 3.7 | 3.4 | 4.3 | 3.2 | 4.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat VEGFR3/FLT4 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 112% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 94.52-190.88 | 100 | 159.39 |
| Rat Serum | 30 | 90.56-139.67 | 100 | 111.39 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat VEGFR3/FLT4 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: VEGFR3/FLT4
VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
