Mouse/Rat HGF enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 154.72 pg/ml (50 μl)；622.09 pg/ml (10 μl) |
| Standard Curve Range | 468.75 ~30000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0110 | 0.0114 | 0.0112 | |
| 468.75 | 0.0184 | 0.0184 | 0.0184 | 0.0072 |
| 937.50 | 0.0326 | 0.0334 | 0.0330 | 0.0218 |
| 1875.00 | 0.0946 | 0.0918 | 0.0932 | 0.0820 |
| 3750.00 | 0.2929 | 0.2983 | 0.2956 | 0.2844 |
| 7500.00 | 0.8757 | 0.8497 | 0.8627 | 0.8515 |
| 15000.00 | 2.0480 | 1.9940 | 2.0210 | 2.0098 |
| 30000.00 | 3.4110 | 3.5690 | 3.4900 | 3.4788 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 666.3 | 3125.1 | 9776.9 | 662.5 | 3043.2 | 9185.5 |
| Standard Deviation | 28.6 | 31.0 | 363.4 | 23.6 | 34.8 | 440.8 |
| Coefficient of Variation(%) | 4.8 | 2.0 | 3.4 | 3.6 | 1.1 | 4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse/rat HGF into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 115% with an overall mean recovery of 88%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | 2.24-20.62 | 100 | 5.52 |
| Rat Serum | 30 | 12.11-51.87 | 100 | 26.58 |
Serum/Plasma – Thirty samples from apparently healthy mice and thirty samples from apparently healthy rats were evaluated for the presence of mouse/rat HGF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: HGF
HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
