Mouse RANKL/TRANCE/TNFSF11 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.36 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0138 | 0.0142 | 0.0140 | |
| 2.74 | 0.0234 | 0.0247 | 0.0241 | 0.0101 |
| 8.23 | 0.0475 | 0.0485 | 0.0480 | 0.0340 |
| 24.69 | 0.1120 | 0.1176 | 0.1148 | 0.1008 |
| 74.07 | 0.3230 | 0.3205 | 0.3218 | 0.3078 |
| 222.22 | 0.9004 | 0.8719 | 0.8862 | 0.8722 |
| 666.67 | 2.2855 | 2.2587 | 2.2721 | 2.2581 |
| 2000.00 | 4.1051 | 3.9973 | 4.0512 | 4.0372 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 60.20 | 211.59 | 633.72 | 62.06 | 191.99 | 568.45 |
| Standard Deviation | 3.50 | 8.89 | 30.71 | 3.23 | 10.65 | 28.61 |
| Coefficient of Variation(%) | 5.8 | 4.2 | 4.8 | 5.2 | 5.5 | 5.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse RANKL/TRANCE/TNFSF11 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 111% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 61.89-141.84 | 100 | 107.38 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of RANKL/TRANCE/TNFSF11 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: RANKL/TRANCE/TNFSF11
TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.
