Mouse Periostin/POSTN enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 5.93 pg/ml (50 μl) |
| Standard Curve Range | 156.25 ~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0172 | 0.0157 | 0.0165 | |
| 156.25 | 0.0644 | 0.0643 | 0.0644 | 0.0479 |
| 312.50 | 0.1121 | 0.1146 | 0.1134 | 0.0969 |
| 625.00 | 0.2067 | 0.2147 | 0.2107 | 0.1943 |
| 1250.00 | 0.4168 | 0.4183 | 0.4176 | 0.4011 |
| 2500.00 | 0.8173 | 0.8154 | 0.8164 | 0.7999 |
| 5000.00 | 1.6130 | 1.6290 | 1.6210 | 1.6046 |
| 10000.00 | 2.9470 | 2.9890 | 2.9680 | 2.9516 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 199.0 | 1022.8 | 3131.7 | 204.4 | 995.0 | 2988.8 |
| Standard Deviation | 9.3 | 44.6 | 138.9 | 9.2 | 34.9 | 120.4 |
| Coefficient of Variation(%) | 4.7 | 4.4 | 4.4 | 4.5 | 3.5 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse Periostin/POSTN into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 113% with an overall mean recovery of 91%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 4.88-10.18 | 100 | 7.37 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of mouse Periostin/POSTN in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Periostin/POSTN
Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
