Mouse IL-9 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.10 pg/ml (10 μl) |
| Standard Curve Range | 1.37~1000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0244 | 0.0248 | 0.0246 | |
| 1.37 | 0.0455 | 0.0463 | 0.0459 | 0.0213 |
| 4.12 | 0.0790 | 0.0843 | 0.0817 | 0.0571 |
| 12.35 | 0.1971 | 0.1957 | 0.1964 | 0.1718 |
| 37.04 | 0.4988 | 0.4858 | 0.4923 | 0.4677 |
| 111.11 | 1.1970 | 1.1910 | 1.1940 | 1.1694 |
| 333.33 | 2.1870 | 2.1790 | 2.1830 | 2.1584 |
| 1000.00 | 2.8400 | 2.8820 | 2.8610 | 2.8364 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 20.5 | 100.5 | 247.8 | 20.0 | 104.3 | 314.7 |
| Standard Deviation | 1.1 | 7.1 | 20.1 | 0.5 | 2.5 | 17.5 |
| Coefficient of Variation(%) | 5.2 | 7.0 | 8.1 | 2.7 | 2.4 | 5.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-9 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 105% to 125% with an overall mean recovery of 117%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.03 | 93 | 0.56 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CXCL1/KC in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-9
Mouse interleukin 9 (IL-9) was originally identified as T cell-derived T cell growth factor III/P40 that could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. Human IL-9 was independently cloned as a novel growth factor that is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% identity at the amino acid level. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.
