Mouse IL-7 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.22 pg/ml (10 μl) |
| Standard Curve Range | 2.74~2000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0106 | 0.0105 | 0.0106 | |
| 20.83 | 0.0248 | 0.0245 | 0.0247 | 0.0141 |
| 41.67 | 0.0517 | 0.0515 | 0.0516 | 0.0411 |
| 83.33 | 0.1378 | 0.1363 | 0.1371 | 0.1265 |
| 166.67 | 0.3766 | 0.3879 | 0.3823 | 0.3717 |
| 333.33 | 1.0650 | 1.0660 | 1.0655 | 1.0550 |
| 666.67 | 2.5270 | 2.5730 | 2.5500 | 2.5395 |
| 2000.00 | 4.2655 | 4.2883 | 4.2770 | 4.2664 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 52.14 | 239.25 | 707.67 | 50.43 | 233.47 | 711.65 |
| Standard Deviation | 2.96 | 9.18 | 26.13 | 1.98 | 11.40 | 36.49 |
| Coefficient of Variation(%) | 5.7 | 3.8 | 3.7 | 3.9 | 4.9 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-7 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 110% with an overall mean recovery of 99%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 0.6-6.24 | 100 | 2.98 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-7 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
