Mouse IL-33 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.33 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0176 | 0.0174 | 0.0175 | |
| 6.86 | 0.0225 | 0.0230 | 0.0228 | 0.0053 |
| 20.58 | 0.0342 | 0.0350 | 0.0346 | 0.0171 |
| 61.73 | 0.0675 | 0.0710 | 0.0693 | 0.0518 |
| 185.19 | 0.1737 | 0.1767 | 0.1752 | 0.1577 |
| 555.56 | 0.5058 | 0.5463 | 0.5261 | 0.5086 |
| 1666.67 | 1.3030 | 1.4530 | 1.3780 | 1.3605 |
| 5000.00 | 3.0570 | 3.1460 | 3.1015 | 3.0840 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 117.2 | 525.0 | 1698.4 | 89.6 | 465.7 | 1468.5 |
| Standard Deviation | 5.8 | 33.7 | 75.0 | 2.8 | 22.1 | 69,2 |
| Coefficient of Variation(%) | 5.0 | 6.4 | 4.4 | 3.2 | 4.7 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IL-33 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 84% to 107% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-2.55 | 53 | 0.53 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-33 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IL-33
IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.
