Mouse IFN-β enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 7.69 pg/ml (10 μl) |
| Standard Curve Range | 46.88~3000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0411 | 0.0365 | 0.0388 | |
| 46.88 | 0.0665 | 0.0600 | 0.0633 | 0.0245 |
| 93.75 | 0.0945 | 0.0893 | 0.0919 | 0.0531 |
| 187.50 | 0.1543 | 0.1501 | 0.1522 | 0.1134 |
| 375.00 | 0.2848 | 0.2850 | 0.2849 | 0.2461 |
| 750.00 | 0.5898 | 0.5864 | 0.5881 | 0.5493 |
| 1500.00 | 1.3820 | 1.2790 | 1.3305 | 1.2917 |
| 3000.00 | 3.2720 | 3.2060 | 3.2390 | 3.2002 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 59.5 | 302.9 | 1001.7 | 73.4 | 302.3 | 911.3 |
| Standard Deviation | 4.2 | 22.4 | 56.9 | 5.7 | 14.5 | 52.5 |
| Coefficient of Variation(%) | 7.0 | 7.4 | 5.7 | 7.7 | 4.8 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse IFN beta into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 106% with an overall mean recovery of 96%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-31.13 | 3.3 | 31.13 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of CRP in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: IFN-β
Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
