Mouse GP130/IL16ST enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 16.07 pg/ml (10 μl) |
| Standard Curve Range | 312.5 ~20000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0301 | 0.0346 | 0.0324 | |
| 312.50 | 0.1019 | 0.1033 | 0.1026 | 0.0703 |
| 625.00 | 0.1807 | 0.1666 | 0.1737 | 0.1413 |
| 1250.00 | 0.3318 | 0.3126 | 0.3222 | 0.2899 |
| 2500.00 | 0.6013 | 0.5963 | 0.5988 | 0.5665 |
| 5000.00 | 0.9942 | 1.0350 | 1.0146 | 0.9823 |
| 10000.00 | 1.9440 | 1.9230 | 1.9335 | 1.9012 |
| 20000.00 | 3.4350 | 3.4580 | 3.4465 | 3.4142 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 510.1 | 2405.5 | 7549.3 | 413.6 | 2054.1 | 5914.5 |
| Standard Deviation | 37.4 | 117.9 | 524.8 | 5.7 | 89.6 | 198.6 |
| Coefficient of Variation(%) | 7.3 | 4.9 | 7.0 | 2.5 | 2.2 | 2.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse GP130/IL16ST into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 100% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 10.56-16.10 | 100 | 13.46 |
Serum/Plasma– Thirty samples from apparently healthy mice were evaluated for the presence of mouse GP130/IL16ST in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: GP130/IL16ST
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
