Mouse CD163 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 15.62pg/ml (10 μl) |
| Standard Curve Range | 156.25 ~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0521 | 0.0460 | 0.0491 | |
| 156.25 | 0.1215 | 0.1155 | 0.1185 | 0.0695 |
| 312.50 | 0.2089 | 0.1872 | 0.1981 | 0.1490 |
| 625.00 | 0.2914 | 0.3162 | 0.3038 | 0.2548 |
| 1250.00 | 0.5026 | 0.5287 | 0.5157 | 0.4666 |
| 2500.00 | 0.8955 | 1.0390 | 0.9673 | 0.9182 |
| 5000.00 | 1.5630 | 1.6870 | 1.6250 | 1.5760 |
| 10000.00 | 2.6230 | 2.8050 | 2.7140 | 2.6650 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| pg/ml | O.D. | Average | Corrected | 0.00 | 0.0521 | |
| Average(pg/ml) | 0.0460 | 0.0491 | 156.25 | 0.1215 | 0.1155 | 0.1185 |
| Standard Deviation | 0.0695 | 312.50 | 0.2089 | 0.1872 | 0.1981 | 0.1490 |
| Coefficient of Variation(%) | 625.00 | 0.2914 | 0.3162 | 0.3038 | 0.2548 | 1250.00 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD163 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 95% to 120% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 185.36-256.77 | 100.0 | 229.54 |
Serum/Plasma– Thirty samples from apparently healthy mice were evaluated for the presence of mouse CD163 in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: CD163
CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.
