Mouse Adiponectin enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.25 ng/ml (10 μl) |
| Standard Curve Range | 1.37~1000 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0070 | 0.0073 | 0.0072 | |
| 1.37 | 0.0102 | 0.0103 | 0.0103 | 0.0031 |
| 4.12 | 0.0159 | 0.0162 | 0.0161 | 0.0089 |
| 12.35 | 0.0354 | 0.0358 | 0.0356 | 0.0285 |
| 37.04 | 0.1021 | 0.0822 | 0.0922 | 0.0850 |
| 111.11 | 0.2987 | 0.2578 | 0.2783 | 0.2711 |
| 333.33 | 0.8970 | 0.7846 | 0.8408 | 0.8337 |
| 1000.00 | 2.4410 | 2.2860 | 2.3635 | 2.3564 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 13.5 | 75.9 | 218.3 | 22.6 | 105.6 | 316.4 |
| Standard Deviation | 0.9 | 5.8 | 13.3 | 0.4 | 2.6 | 7.1 |
| Coefficient of Variation(%) | 6.7 | 7.6 | 6.1 | 1.9 | 2.4 | 2.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse Adiponectin into health Mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1.61-27.96 | 100 | 15.44 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of Coagulation Factor XII in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Adiponectin
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.
