Mammal NF-L enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.54 pg/ml (50 μl)；1.31 pg/ml (10 μl) |
| Standard Curve Range | 6.86~5000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0058 | 0.0061 | 0.0060 | |
| 6.86 | 0.0192 | 0.0156 | 0.0174 | 0.0115 |
| 20.58 | 0.0364 | 0.0350 | 0.0357 | 0.0298 |
| 61.73 | 0.0947 | 0.0897 | 0.0922 | 0.0863 |
| 185.19 | 0.2678 | 0.2753 | 0.2716 | 0.2656 |
| 555.56 | 0.7753 | 0.7460 | 0.7607 | 0.7547 |
| 1666.67 | 2.0194 | 1.9554 | 1.9874 | 1.9815 |
| 5000.00 | 3.9390 | 3.9208 | 3.9299 | 3.9240 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 53.0 | 385.7 | 1612.4 | 127.7 | 547.7 | 1800.6 |
| Standard Deviation | 2.2 | 17.4 | 76.9 | 6.7 | 25.3 | 78.4 |
| Coefficient of Variation(%) | 4.1 | 4.5 | 4.8 | 5.2 | 4.6 | 4.4 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammal NF-L into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-1.18 | 25 | 0.55 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NF-L in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: NF-L
NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that is expressed in neurons. It associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.
