Mammalian Insulin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 20.89 pg/ml (50 μl) ；28.28 pg/ml (20 μl) |
| Standard Curve Range | 31.25~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/20 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0081 | 0.0076 | 0.0079 | |
| 31.25 | 0.0100 | 0.0101 | 0.0101 | 0.0022 |
| 62.50 | 0.0122 | 0.0127 | 0.0125 | 0.0046 |
| 125.00 | 0.0245 | 0.0242 | 0.0244 | 0.0165 |
| 250.00 | 0.0767 | 0.0754 | 0.0761 | 0.0682 |
| 500.00 | 0.3493 | 0.3107 | 0.3300 | 0.3222 |
| 1000.00 | 1.2640 | 1.1620 | 1.2130 | 1.2052 |
| 2000.00 | 3.2150 | 3.1260 | 3.1705 | 3.1627 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 581.0 | 2316.1 | 7446.9 | 579.8 | 2277.0 | 7736.2 |
| Standard Deviation | 12.8 | 34.2 | 243.7 | 14.4 | 34.5 | 230.5 |
| Coefficient of Variation(%) | 2.2 | 1.5 | 3.3 | 2.5 | 1.5 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested Twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammalian Insulin into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 110%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Mouse Serum | 30 | n.d-289.05 | 90 | 41.29 |
| Rat Serum | 30 | 13.76-924.50 | 100 | 106.12 |
| Human Serum | 30 | 132.03-2758.63 | 100 | 1027.08 |
Serum/Plasma – Thirty samples from apparently healthy mice/healthy volunteers/healthy rats were evaluated for the presence of Mammalian Insulin in this assay. No medical histories were available for the donors.n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: Insulin
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.
