Mammal GFAP enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.52 pg/ml (50 μl)；17.06 pg/ml (10 μl)； |
| Standard Curve Range | 68.59~50000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0335 | 0.0361 | 0.0348 | |
| 68.59 | 0.0570 | 0.0603 | 0.0587 | 0.0239 |
| 205.76 | 0.0843 | 0.0939 | 0.0891 | 0.0543 |
| 617.28 | 0.1807 | 0.1806 | 0.1807 | 0.1459 |
| 1851.85 | 0.4176 | 0.4205 | 0.4191 | 0.3843 |
| 5555.56 | 1.1570 | 1.1750 | 1.1660 | 1.1312 |
| 16666.67 | 2.8440 | 2.9960 | 2.9200 | 2.8852 |
| 50000.00 | 4.3945 | 4.5339 | 4.4642 | 4.4294 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 724.3 | 3800.8 | 14128.0 | 794.5 | 4527.5 | 16907.8 |
| Standard Deviation | 2.2 | 17.4 | 76.9 | 6.7 | 25.3 | 78.4 |
| Coefficient of Variation(%) | 4.7 | 6.5 | 5.2 | 3.8 | 5.4 | 5.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammal GFAP into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.29 | 75 | 5.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of GFAP in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: GFAP
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.
