Mammal BACE-1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 5.24 pg/ml (50 μl)；6.31 pg/ml (10 μl)； |
| Standard Curve Range | 156.25~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0169 | 0.0149 | 0.0159 | |
| 156.25 | 0.1009 | 0.0997 | 0.1003 | 0.0844 |
| 312.50 | 0.1989 | 0.1893 | 0.1941 | 0.1782 |
| 625.00 | 0.4168 | 0.4181 | 0.4175 | 0.4016 |
| 1250.00 | 1.0129 | 0.9899 | 1.0014 | 0.9855 |
| 2500.00 | 2.1601 | 2.1772 | 2.1687 | 2.1528 |
| 5000.00 | 3.8309 | 3.7322 | 3.7816 | 3.7657 |
| 10000.00 | 4.3801 | 4.5571 | 4.4686 | 4.4527 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 162.5 | 853.2 | 3374.8 | 165.2 | 827.7 | 3388.4 |
| Standard Deviation | 9.3 | 31.9 | 119.4 | 9.4 | 16.8 | 172.8 |
| Coefficient of Variation(%) | 5.7 | 3.7 | 3.5 | 5.7 | 2.0 | 5.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammal BACE-1 into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 113% with an overall mean recovery of 104%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 567.43-956.16 | 100 | 713.37 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of BACE-1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: BACE-1
BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein. It is the major beta secretase, and together with the gamma secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms. In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins.
