Human VEGFR1/VEGF Receptor/FLT1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.11 pg/ml (50 μl)；4.51 pg/ml (10 μl)； |
| Standard Curve Range | 31.25 ~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0426 | 0.0404 | 0.0415 | |
| 31.25 | 0.1325 | 0.1354 | 0.1340 | 0.0925 |
| 62.50 | 0.2231 | 0.2240 | 0.2236 | 0.1821 |
| 125.00 | 0.3932 | 0.3935 | 0.3934 | 0.3519 |
| 250.00 | 0.7340 | 0.7318 | 0.7329 | 0.6914 |
| 500.00 | 1.3310 | 1.3110 | 1.3210 | 1.2795 |
| 1000.00 | 2.4640 | 2.4440 | 2.4540 | 2.4125 |
| 2000.00 | 4.0933 | 4.0831 | 4.0882 | 4.0467 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 36.8 | 222.2 | 722.0 | 38.5 | 227.9 | 748.0 |
| Standard Deviation | 1.6 | 7.6 | 31.5 | 0.7 | 2.2 | 7.1 |
| Coefficient of Variation(%) | 4.5 | 3.4 | 4.4 | 1.8 | 1.0 | 0.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human VEGFR1/VEGF Receptor/FLT1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 82% to 125% with an overall mean recovery of 107%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 243.29-1159.18 | 100 | 522.74 |
Serum/Plasma– Thirty samples from apparently healthy volunteers/mouse serum were evaluated for the presence of human VEGFR1/VEGF Receptor/FLT1 in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: VEGFR1/VEGF Receptor/FLT1
Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.
