Human TRAF1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 2.85 pg/ml (50 μl)；7.62 pg/ml (10 μl)； |
| Standard Curve Range | 20.48~5000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0352 | 0.0374 | 0.0363 | |
| 20.48 | 0.0482 | 0.0512 | 0.0497 | 0.0134 |
| 51.20 | 0.0685 | 0.0666 | 0.0676 | 0.0313 |
| 128.00 | 0.1148 | 0.1100 | 0.1124 | 0.0761 |
| 320.00 | 0.2349 | 0.2201 | 0.2275 | 0.1912 |
| 800.00 | 0.5329 | 0.5233 | 0.5281 | 0.4918 |
| 2000.00 | 1.2500 | 1.2230 | 1.2365 | 1.2002 |
| 5000.00 | 2.8350 | 2.7000 | 2.7675 | 2.7312 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 89.7 | 437.9 | 1342.2 | 79.1 | 414.5 | 1371.4 |
| Standard Deviation | 4.9 | 16.7 | 58.5 | 5.9 | 8.8 | 47.9 |
| Coefficient of Variation(%) | 5.5 | 3.8 | 4.4 | 7.4 | 2.1 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TRAF1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from72% to 94% with an overall mean recovery of 84%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | n.d.-43.04 | 63 | 14.45 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TRAF1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TRAF1
TRAF1（肿瘤坏死因子受体相关因子1）是一种调控炎症与细胞凋亡的细胞内衔接蛋白
