Human TNF beta/Lymphotoxin alpha enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 10.59 pg/ml (50 μl)；83.08 pg/ml (10 μl)； |
| Standard Curve Range | 40.96~10000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0139 | 0.0137 | 0.0138 | |
| 40.96 | 0.0270 | 0.0261 | 0.0266 | 0.0128 |
| 102.40 | 0.0495 | 0.0454 | 0.0475 | 0.0337 |
| 256.00 | 0.1006 | 0.0943 | 0.0975 | 0.0837 |
| 640.00 | 0.2308 | 0.2137 | 0.2223 | 0.2085 |
| 1600.00 | 0.5287 | 0.4955 | 0.5121 | 0.4983 |
| 4000.00 | 1.2490 | 1.1550 | 1.2020 | 1.1882 |
| 10000.00 | 2.4840 | 2.4060 | 2.4450 | 2.4312 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 326.8 | 1415.9 | 4105.8 | 226.7 | 1146.4 | 3807.0 |
| Standard Deviation | 21.7 | 86.6 | 262.9 | 8.1 | 65.9 | 68.6 |
| Coefficient of Variation(%) | 6.6 | 6.1 | 6.4 | 3.6 | 5.7 | 1.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF beta/Lymphotoxin alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 117% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-31.50 | 25 | 16.64 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TNF beta/Lymphotoxin alpha in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TNF beta/Lymphotoxin alpha
Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta. Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14. LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3. In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function. LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease.
