Human TIM4 enzyme-linked immunoassay kit(one step)
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.001 ng/ml (50 μl)；0.03 ng/ml (10 μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0066 | 0.0066 | 0.0066 | |
| 0.14 | 0.0584 | 0.0587 | 0.0586 | 0.0520 |
| 0.41 | 0.1645 | 0.1618 | 0.1632 | 0.1566 |
| 1.23 | 0.4574 | 0.4633 | 0.4604 | 0.4538 |
| 3.70 | 1.2320 | 1.2350 | 1.2335 | 1.2269 |
| 11.11 | 2.6720 | 2.6900 | 2.6810 | 2.6744 |
| 33.33 | 3.4720 | 3.4610 | 3.4665 | 3.4599 |
| 100.00 | 3.7210 | 3.6020 | 3.6615 | 3.6549 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 1.7 | 12.8 | 30.6 | 1.6 | 13.8 | 31.6 |
| Standard Deviation | 0.1 | 1.1 | 4.1 | 0.1 | 1.5 | 5.2 |
| Coefficient of Variation(%) | 3.1 | 4.5 | 3.2 | 2.7 | 3.9 | 4.2 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TIM4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 113% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 4.94-10.86 | 100 | 7.18 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TIM4 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: TIM4
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain . The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells.
