Human SAA1 enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.30pg/ml (50 μl)；1.56 pg/ml (10 μl)； |
| Standard Curve Range | 1.56~100 ng/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0226 | 0.0227 | 0.0227 | |
| 1.56 | 0.0334 | 0.0370 | 0.0352 | 0.0126 |
| 3.13 | 0.0579 | 0.0619 | 0.0599 | 0.0373 |
| 6.25 | 0.1442 | 0.1511 | 0.1477 | 0.1250 |
| 12.50 | 0.3880 | 0.4018 | 0.3949 | 0.3723 |
| 25.00 | 1.2466 | 1.2545 | 1.2506 | 1.2279 |
| 50.00 | 3.1485 | 3.0604 | 3.1045 | 3.0818 |
| 100.00 | 4.6630 | 4.7992 | 4.7311 | 4.7085 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average() | 2.4 | 12.0 | 38.0 | 2.5 | 11.4 | 35.1 |
| Standard Deviation | 0.1 | 0.4 | 1.3 | 0.1 | 0.5 | 1.3 |
| Coefficient of Variation(%) | 5.6 | 3.0 | 3.5 | 2.9 | 4.1 | 3.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human SAA1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 117% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range () | Detectable (%) | Mean of Detectable () |
|---|---|---|---|---|
| Serum | 30 | 24.57-213.30 | 100 | 118.34 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SAA1 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: SAA1
Mouse serum amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to pro-inflammatory cytokines. It is secreted as a 12 kDa, 103 amino acid (aa), nonglycosylated polypeptide and circulates as part of the HDL complex. The SAA1 gene is one of three SAA genes in mouse, and, based on human, is likely to be allelic. The SAA1 gene product differs from the SAA2 gene product by only nine amino acids. In human, circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one to three amino acids being cleaved from either the N- or C-terminus. The same situation may exist in mouse. The amino acid sequence of mature mouse SAA1 is 72%, 72%, 67%, and 74% identical to mature human, rabbit, equine, and hamster SAA1, respectively.
