Human Resistin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.33 pg/ml (50 μl)；0.48 pg/ml (10 μl)； |
| Standard Curve Range | 13.72~10000 pg/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0118 | 0.0113 | 0.0116 | |
| 13.72 | 0.0165 | 0.0175 | 0.0170 | 0.0055 |
| 41.15 | 0.0270 | 0.0278 | 0.0274 | 0.0159 |
| 123.46 | 0.0605 | 0.0604 | 0.0605 | 0.0489 |
| 370.37 | 0.1887 | 0.1819 | 0.1853 | 0.1738 |
| 1111.11 | 0.5823 | 0.5926 | 0.5875 | 0.5759 |
| 3333.33 | 1.9340 | 1.8680 | 1.9010 | 1.8895 |
| 10000.00 | 4.2792 | 4.3434 | 4.3113 | 4.2998 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 173.5 | 899.9 | 3107.1 | 179.8 | 932.5 | 3171.0 |
| Standard Deviation | 6.8 | 32.8 | 125.6 | 3.0 | 17.6 | 149.7 |
| Coefficient of Variation(%) | 3.9 | 3.6 | 4.0 | 1.7 | 1.9 | 4.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Resistin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 116% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 0.81-34.93 | 100 | 18.17 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Resistin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Resistin
Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.
