Human RBP4 enzyme-linked immunoassay kit（one step）
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.002 ng/ml (50 μl)；0.03 ng/ml (10 μl)； |
| Standard Curve Range | 0.14~100 ng/ml |
| Standard Curve Gradient | 7 Points/3 Folds |
| Number of Incubations | 2 |
| Detectable sample | serum, plasma |
| Sample Volume | 50 μl/10 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 60min |
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0087 | 0.0084 | 0.0086 | |
| 0.14 | 0.0340 | 0.0339 | 0.0340 | 0.0254 |
| 0.41 | 0.0873 | 0.0877 | 0.0875 | 0.0790 |
| 1.23 | 0.2276 | 0.2253 | 0.2265 | 0.2179 |
| 3.70 | 0.6074 | 0.5874 | 0.5974 | 0.5889 |
| 11.11 | 1.4550 | 1.3330 | 1.3940 | 1.3855 |
| 33.33 | 3.1820 | 3.2770 | 3.2295 | 3.2210 |
| 100.00 | 4.0330 | 4.2409 | 4.1370 | 4.1284 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(ng/ml) | 1.2 | 9.1 | 33.5 | 1.3 | 12.6 | 34.1 |
| Standard Deviation | 0.1 | 0.5 | 2.5 | 0.1 | 0.4 | 1.4 |
| Coefficient of Variation(%) | 4.9 | 5.8 | 5.4 | 5.9 | 3.1 | 4.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human RBP4 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 88% to 118% with an overall mean recovery of 100%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 278.09-774.24 | 100 | 534.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of RBP4 in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: RBP4
Retinol-binding protein 4 (RBP4) is a Lipocalin superfamily molecule that transports vitamin A (retinol) and retinaldehyde in the serum. Retinaldehyde is the critical chromophore in the rhodopsin photoreceptor, while both it and retinoic acid regulate a multitude of cellular differentiation and proliferation effects through the intracellular receptors RAR and RXR. RBP4 is secreted primarily by hepatocytes and adipocytes into the blood where the RBP4-retinol complex interacts with transthyretin (TTR). Formation of the complex with TTR increases the serum half-life of RBP4 by preventing RBP4 filtration through the kidney. The C-terminally processed forms of RBP4, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. RBP4 promotes hyperglycemia, and its upregulation in visceral and liver adipocytes leads to elevated serum levels.
