Human Prolactin enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 3.47 pg/ml (50 μl)；11.20 pg/ml (10 μl) |
| Standard Curve Range | 31.25~2000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Sample Volume | 50 μl/10 μl |
| Type | Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0672 | 0.0630 | 0.0651 | |
| 31.25 | 0.1314 | 0.1212 | 0.1263 | 0.0612 |
| 62.50 | 0.1703 | 0.1765 | 0.1734 | 0.1083 |
| 125.00 | 0.3135 | 0.3042 | 0.3089 | 0.2438 |
| 250.00 | 0.5341 | 0.5419 | 0.5380 | 0.4729 |
| 500.00 | 1.0220 | 1.0100 | 1.0160 | 0.9509 |
| 1000.00 | 1.9160 | 1.9370 | 1.9265 | 1.8614 |
| 2000.00 | 3.3550 | 3.4380 | 3.3965 | 3.3314 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 38.9 | 216.2 | 558.7 | 43.9 | 229.2 | 598.9 |
| Standard Deviation | 3.2 | 6.4 | 16.7 | 3.3 | 8.4 | 18.1 |
| Coefficient of Variation(%) | 8.0 | 2.9 | 3.0 | 7.4 | 3.7 | 3.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Prolactin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 115% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1055.28-7606.60 | 100 | 3687.09 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Prolactin in this assay. No medical histories were available for the donors.
Product Data Sheet
Background: Prolactin
Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.
Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.
