Human PLGF enzyme-linked immunoassay kit
| Specification | 96 Test |
|---|---|
| Sensitivity | 0.83 pg/ml (50 μl) |
| Standard Curve Range | 4.10~1000 pg/ml |
| Standard Curve Gradient | 7 Points |
| Number of Incubations | 2 |
| Detectable sample | Liquid phase sample of soluble substances. For example: serum, plasma, cell culture supernatant, tissue grinding liquid, etc. |
| Sample Volume | 50 μl |
| Type | Fully Ready-to-Use |
| Operation Duration | 120min |
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0377 | 0.0413 | 0.0395 | |
| 4.10 | 0.0677 | 0.0737 | 0.0707 | 0.0312 |
| 10.24 | 0.1165 | 0.1256 | 0.1211 | 0.0816 |
| 25.60 | 0.2566 | 0.2508 | 0.2537 | 0.2142 |
| 64.00 | 0.5458 | 0.5588 | 0.5523 | 0.5128 |
| 160.00 | 1.1780 | 1.1940 | 1.1860 | 1.1465 |
| 400.00 | 2.4310 | 2.4210 | 2.4260 | 2.3865 |
| 1000.00 | 3.9734 | 3.8360 | 3.9047 | 3.8652 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 17.7 | 97.2 | 294.9 | 17.4 | 92.3 | 284.1 |
| Standard Deviation | 0.8 | 3.7 | 11.0 | 0.8 | 4.2 | 10.2 |
| Coefficient of Variation(%) | 4.5 | 3.8 | 3.7 | 4.7 | 4.6 | 3.6 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty-two times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human PLGF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 109% with an overall mean recovery of 93%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| serum | 30 | 7.82-29.54 | 100.0 | 14.90 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human PLGF in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Product Data Sheet
Background: PLGF
PlGF (placenta growth factor) is secreted primarily by villous trophoblasts and decidual cells, with circulating levels increasing during pregnancy but attenuated in preeclampsia. PlGF signals through VEGF R1/Flt‑1 and Neuropilins, while VEGF binds both VEGF R1 and R2 but signals mainly through VEGF R2. PlGF reduces binding of VEGF to VEGF R1 and increases VEGF/VEGF R2‑mediated angiogenesis. It induces monocyte activation, migration, and production of inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture healing and also contribute to inflammation in active sickle cell disease and atherosclerosis.
